Simulation and Experimental Study on the Responses of Subcellular Structures in Tumor Cells Induced by 5 ns Pulsed Electric Fields

In order to explore the bioelectric effect of 5 ns pulsed electric fields on tumor cells, a spherical single-cell multiphysics model was first established based finite element simulation platform. consideration dielectric relaxation biological plasma membrane under high-frequency fields, electroporation and Maxwell stress tensors cell nuclear envelope were analyzed; secondly, taking MDA-MB-231 cells as research object, combined with fluorescent probe technology, state change fluorescence dissipation its subcellular structure exposed pulse studied. The results showed that directly acted inside cell, causing an tensile envelope, destroying integrity cytoskeleton, damaging functions structures including endoplasmic reticulum, mitochondria, etc. This study provides theoretical experimental evidence for application high-voltage short in field biomedical engineering.